Fig. 4. Northern blot hybridization of uterine Hegfl mRNA in steroid-treated adult ovariectomized hamsters. The expression of Hegfl mRNA was detected in total RNA samples obtained from whole ovariectomized hamster uteri after various steroid treatments. Ovariectomized hamsters were given a single injection of progesterone (P₄), estradiol-17ß (E₂) or P₄ plus E₂. Control ovariectomized hamsters received an injection of oil (0 hour). Uteri were collected at the indicated hour (h) after steroid treatments. As mentioned in the Fig. 1, an approx. 2.5 kb transcript for Hegfl was detected in the uterus. The blot was hybridized with Rpl7 to confirm integrity, equal loading, and blotting of RNA samples. These experiments were repeated twice with similar results. Bar diagram above the autoradiogram shows the relative levels of Hegfl mRNA (Hegfl mRNA/Rpl7 mRNA) after densitometric scanning of this autoradiogram.

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