Fig. 3. CD34⁺ CD45⁺ and CD34⁺CD45^– cell sorting from the 40-day embryonic liver and re-analysis by flow cytometry. Dissociated cells were double-stained with FITC-anti-CD34 and PE-anti-CD45 MoAbs. CD34⁺ CD45⁺ and CD34⁺ CD45^– cell subsets were sorted inside the gates (A) and immediately re-analyzed (B,C). A fraction of each sorted cell subset was then incubated, first, with biotinylated UEA-1 and then with APC-conjugated streptavidin. All sorted CD34⁺ CD45^– cells were stained by UEA-1 (E), whereas none of the CD34⁺ CD45⁺ cells was (D).

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