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Fig. 6. Analysis of down-stream signaling targets of PTEN controlled pathway. (A) Northern blot analysis of ß-casein, cyclin D1, and c-myc mRNA levels in control (C) and mutant (M) mammary glands. RNA was extracted from pregnancy day 13 (P13), lactation day 10 (L10), and involution day 1, 3 and 6 glands, respectively (I-1 to I-6). One representative of two independent experiments is shown. The amount of ribosomal RNAs was used as loading control. (B-E) Western blot analysis. Total cell lysates from lactation day 10 (L10), involution day 1 (I-1), day 3 (I-3), and day 6 (I-6) glands were examined. Mutant and control are labeled as M, mutant; C, control. {alpha}-actin antibody was used as loading control.





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