Fig. 1. Interactions between Ring1B and Mel18. (A) Interaction in yeast cells indicated by the ß-galactosidase activity. Yeast cellswere transformed with the indicated plasmids. Lamin was a negative control. (B) GST pull-down assays. (Part a) Binding of ³⁵S-labeled full-length Mel18 protein (Mel18fl) to GST or GST fused to either full-length or truncated Ring1B (GST-Ring1Bfl, GST-Ring1BC or GST-Ring1BN, respectively). (Part b) Binding of ³⁵S-labeled full-length Ring1B protein (Ring1Bfl) to GST or GST fused to truncated Mel18 (GST-Mel18PEST) (amino acids 1-233). (Part c) Binding of ³⁵S-labeled truncated Ring1B proteins (Ring1BC and Ring1BN) to GST or GST-Mel18PEST.

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