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Fig. 4. Subcellular localization of RING1B in interphase and mitotic nuclei. (A,B) Distribution of RING1B and CENPB (A) and centromeric antigens (B) in interphase nucleus of U2-OS cells. (Parts a) Localization of endogenous RING1B (green). (Parts b) Localization of endogenous CENPB (A) and centromeric antigens (B; ACA) (red). (Parts c) DAPI (blue). (Parts d) Merged images. (Parts e) Higher magnification views of the boxed regions in parts d. (C) Distribution of RING1B (part a, green) and CENPB (part b, red) in prophase nucleus of U2-OS cells. (part c) DAPI (blue). (Part d) Merged images. (D) Distribution of RING1B (part a; green) and centromeric antigens (part b; red) in prometaphase nucleus of U2-OS cells. (Part c) DAPI (blue). (Part d) Merged images. (Part E) Distribution of RING1B (part a; green) and CENPB (part b; red) in anaphase nucleus of U2-OS cells. (Part c) DAPI (blue). (Part d) Merged images.





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