Fig. 4. Capping of ConA receptors. Amoebae, preincubated either at 22°C or 28°C, were labelled with Fl-ConA for 5 minutes at the appropriate temperature, washed and incubated a further 5 minutes, fixed with formaldehyde and viewed according to Aguado-Velasco and Bretscher (Aguado-Velasco and Bretscher, 1997). The fraction of capped cells, measured as those with the fluorescence confined to one half of the cell surface, was: at 22°C, Ax2, 76±5%; ts1, 68±2%; ts2, 77±4%; and at 28°C, Ax2, 79±2%; ts1, 0%; ts2, 0%.

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