Fig. 2. SoxN mutation leads to the loss of lateral and intermediate neuroblasts. (A-E) Immunostaining with anti-Wor antibody. (A) Wild-type and (B) SoxN stage 9 embryos; (A',B') higher magnification of two consecutive segments of A,B. The broken line indicates the ventral midline; (v) ventral, (i) intermediate and (l) lateral column of NBs. The arrowhead marks NB5-3 (A') or the position of NB5-3 (B'); the asterisk indicates the position of NB3-5. (C) Quantification of the SI NB phenotype: the percentages of samples showing loss of each NB are given. Note that the loss of NBs predominantly occurs in the intermediate and lateral column. (D) Wild-type and (E) SoxN stage 11 embryos. Note the drastic loss of NBs in E. (F) Wild-type and (G) SoxN stage 11 embryos stained with anti-Eagle antibody. Note the complete absence of Eagle-positive NBs in G. (H) Wild-type and (I) SoxN stage 11 embryos stained with anti-ß-gal to detect hkb-lacZ (5953) expression (black) and anti-Engrailed (brown) to facilitate the identification of positions along the AP axis. (I) Note the strong reduction of hkb-lacZ expression in the intermediate and lateral regions of the neuroectoderm. (H',I') Higher magnification of one hemisegment of H,I. Note the loss of NB2-4 in row 2 and the complete absence of hkb-lacZ expression in row 4. Ventral views with anterior towards the left.

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