Fig. 1. Prop1 and Lhx4 are necessary for normal temporal and spatial activation of Lhx3 and Isl1. (A-D) Embryos collected at E12.5 were genotyped as described in Materials and Methods, sectioned in the sagittal plane, and stained with Hematoxylin and Eosin (H&E). (E-P) Immunohistochemistry was used to detect LHX3 (E-H), ISL1 (I-L), and PITX1 (M-P) in sections from each genotype. LHX3 is present throughout Rathke’s pouch in wild-type and Prop1^df/df mice (E,F), but only a few LHX3-positive cells were present in Lhx4 mutants (arrow and inset, G), and no positive cells were detected in double mutants (H). (I-L) ISL1 is restricted to the ventral part of the anterior lobe in wild-type and Prop1^df/df mice (I,J) but it is expressed in the most dorsal area of Lhx4 mutant pituitaries (K) and in double mutants (L). (M-P) PITX1 is present in the pituitaries of all the genotypes examined.

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