Fig. 5. Cxcr4 mutant mice had ectopic dentate granule cells. Prox1 immunohistochemistry in the E18.5 hippocampus of Cxcr4^+/– and Cxcr4^–/– mice. Scale bar: 250 µm. (A,B) In Cxcr4^+/– mice, Prox-1 positive granule cells were in the dentate gyrus. In Cxcr4^–/– mice many Prox1-positive granule cells were in the dentate gyrus, but appeared disorganized. Additionally, many Prox1-positive cells were outside the dentate gyrus, near the ventricular zone, above the fimbria and in the dentate migratory stream (arrows). (C,D) In Cxcr4^+/– mice Phosphohistone-H3 positive precursor cells were distributed in the dentate subventricular zone, migratory stream and in the tertiary matrix. In addition, note the scattered positive cells throughout the ventricular zone of the hippocampus. In Cxcr4^–/– mice the number of dividing precursor cells labeled with Phosphohistone-H3 was dramatically decreased in all the regions but is preserved outside the dentate gyrus in the ventricular zone. (E,F) Nestin antibody staining in Cxcr4^+/– and Cxcr4^–/– mice showing that the overall distribution of the radial glial network is intact.

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