Fig. 3. DV differential effects of tsh misexpression. (a) bi>GFP marks bi-GAL4 expression domains along the dorsal and ventral margins of the eye disc, and in a dorsal sector of the antennal disc. The DV axis (as defined by the ventral wg and dorsal dpp expression) of the antenna disc (Theisen et al., 1996) is reversed from that of the eye disc. (b) bi>tsh caused eye suppression and ectopic HTH (red) induction only in the ventral eye margin (arrow) of the eye disc. The eye disc is also enlarged. (c) bi>tsh pharate adult showed ventral eye suppression (arrow) and dorsal eye overgrowth. (d) bi>hth adult showed eye suppression on both dorsal and ventral eye (arrows). The adult eye phenotype was usually more severe than the disc phenotype. (e) Act>tsh clone (marked by GFP: green) induced HTH (red) autonomously and suppressed eye development (ELAV: blue) in the ventral margin of the eye disc (arrowhead). Clone in the dorsal margin did not induce HTH but caused overgrowth (arrow). Act>tsh clones were not marked in adults, but flies with clone induction showed ventral suppression (f, arrow) or dorsal enlargement (g, arrow) in the eye.

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