Fig. 3. Expression of P-INO reporter genes in sup-5. Stages: (A,F,K,P) 2-II; (B,G,L,Q) 2-IV; (C,H,M,R) 2-V; (D,I,N,S) 3-I; (E,J,O,T) 4-I. (A-E) Scanning electron micrographs of sup-5 ovules. (F-J) Composite figures of DIC and confocal INO:GFP fluorescence images and (K-O) respective INO:GFP fluorescence alone in sup-5 ovules. Although P-INO::INO:GFP::NOS3' fluorescence is limited to a single cell layer, it expands to the adaxial side of the ovule primordium. (M,N) Apparent internal localization of INO:GFP is from glancing sections through adjacent surface layers. (P-T) DIC images of sup-5 ovules stained for P-INO::GUS::INO3' activity using 12.5 µg/ml X-gluc. In all panels, the abaxial side of the ovule is to the left. n, nucellus; i, inner integument; f, funiculus; o, outer integument; m, micropyle; arrowhead, outer integument primordium. Scale bar: 25 µm in A-C,F-H,K-M,P-R; 30 µm in D,I,N,S; 50 µm in E,J,O,T.

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