Fig. 4. Effects of P-INO::SUP::INO3' and P-INO::CRC::INO3' transgenes on ovule morphology and reporter gene expression. Stages: (A,F,K,P) 2-III; (B,G) early 2-IV; (C,H,L,Q) 2-IV; (D,I,M,R) 2-V; (N,S) 3-I; (E,J,O,T) 4-I. (A-E) Scanning electron micrographs of ovules from wild-type plants containing the P-INO::SUP::INO3' transgene. (F-J) DIC images of P-INO::SUP::INO3' ovules stained for P-INO::GUS::INO3' activity using 125 µg/ml X-gluc. (K-O) Scanning electron micrographs of ovules from ino-1 plants containing the P-INO::CRC::INO3' transgene. (P-T) DIC images of P-INO::CRC::INO3' ovules from ino-1 plants stained for P-INO::GUS::INO3' activity using 12.5 µg/ml X-gluc. In all panels, the abaxial side of the ovules is to the left. n, nucellus; i, inner integument; f, funiculus; o, outer integument; m, micropyle; arrowhead, outer integument primordium. Scale bar: 25 µm in A-D,F-I,K-M,P-R; 30 µm in N,S; 50 µm in E,J,O,T.

Return to article