Fig. 3. Expression pattern of INO during ovule development in nzz-2 (A-D), ats (E,F), sup-5 (G,H) and nzz-2 ats (I-L). Stages: (A,G,I) 1-II/2-I; (B,E,H,J) 2-III; (C) 2-IV; (D,F,K) 3-I; (L) 3-IV. INO expression remains unaltered in nzz-2 except that it might be shifted distally by few cells as observed by the shifting of the outer integument (A-D). The tip cell does not show INO expression as in wild type (B arrow). The absence of INO expression at the adaxial side can be seen in ats mutants (arrow in E). The tip cell and the adaxial cell layer of the outer integument do not show INO expression in ats mutants (arrow in F). In sup-5 early onset of INO expression is similar to wild type (arrow in G), but around stage 2-III, INO expression can be detected throughout the central region (arrow in H). In ovules of nzz-2 ats mutants, INO expression is similar to wild type in most instances (arrow in I,a), but sometimes the expression can be seen throughout the central region (arrow in I,b). Instead of a ‘horseshoe appearance’ a complete ring can be observed in a cross section through the central region of a nzz-2 ats double mutant (arrow in L,a). Scale bars: 20 µm.

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