Fig. 2. The subepidermal layer of scz mutants develops root hairs and expresses epidermal cell specific markers. (A) Median longitudinal optical section through the scz mutant root showing two cells with root hairs arising from subepidermal cell layers. (B) Transverse sections through the root shown in A at positions indicated by arrows illustrate that the root hair originates from the cortex (asterisk). (C,D) Expression of GFP-enhancer trap line J0481 in the differentiation zone of a scz mutant root (C) and wild-type root (D). Median longitudinal sections stained with propidium iodide are shown on the left, GFP expression (green) is in the middle and superimposed images are shown on the right. The enhancer trap is expressed in the epidermis and ground tissue in scz mutants (C), in contrast to wild type where expression is restricted to the epidermis (D). Bar, 25 µm.

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