Fig. 5. Cyp26 can suppress posterior genes but does not induce anterior genes in the posterior region. Dorsal views of whole embryos at late gastrula stage. cyp26 mRNA was injected at the two-cell stage into one blastomere. Some of the embryos were treated with RA (K,L). Embryos were stained for hoxb1b (A,B), meis3 (C,D), iro1 (E,F), otx2 (G,H,K,L) and otx2/hoxb1b together (I,J). hoxb1b, meis3 and the posterior domain of iro1 were suppressed by cyp26 injection (B,D,F,J, arrowhead), but otx2 and the anterior domain of iro1 expression were neither suppressed nor expanded (F,H,J). The suppression of otx2 by RA was partially rescued by cyp26 (L, arrowhead).

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