Fig. 7. Native Dlx binding sites. (A) Dlx1, Dlx2 and Dlx5 binding sites in the Dlx5/Dlx6 intergenic region (Zerucha et al., 2000). (B) Dlx2 binding site in Wnt1 (Iler et al., 1995). This interaction occurs predominantly via HBS-1 and the result is repression, not activation. (C) Xenopus Dlx3-binding site in human profilaggrin promoter (Morasso et al., 1996). (D) Dlx3-binding site in gene encoding the alpha subunit of human chorionic gonadotropin (Roberson et al., 2001). (E) Dlx5-binding site in collagen 1A1 (Dodig et al., 1996). (F) Dlx5 binding site in osteocalcin (Ryoo et al., 1997). (G) Dlx5-binding site in bone sialoprotein (Benson et al., 2000). We note that in several of these native binding sites, there are pairs of 5'-TAATT-3' sequences on opposite strands, and propose that these might bind Dlx dimers. Intriguingly, in vivo site selection with Dlx3 recovered opposing 5'-TAATT-3' sequences in 17/30 selected sites (Feledy et al., 1999b). We also note that the WIP sequence, which mediates repression by Dlx2, and OC-Box 1, which initially was reported to mediate repression, have 5'-CAAT-3' instead of 5'-TAATT-3' opposing a 5'-TAATT-3'.

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