Fig. 3. CSN4 and CSN5 are essential for oogenesis. Ovaries (A-C), egg chambers (F-H), and the most developed stage oocytes (D,E,I-L) are shown for the following genotypes: wild type (A,D); w/w hs-FLP; FRT csn4⁺/FRT OVO^D, heat-shocked (E); w/w hs-FLP; FRT csn4^null/FRT OVO^D, non heat-shocked (H,I); w/w hs-FLP; FRT csn4^null/FRT OVO^D, heat-shocked (B,F,J); w/w hs-FLP; FRT csn5^null/FRT OVO^D, heat-shocked (G,K); w/w hs-FLP; csn5^P/FRT OVO^D, heat-shocked (C,L). (A,D) Wild-type control; (E) negative control showing that heat shock does not effect oogenesis; (H,I) OVO^D-derived oocytes. (B,C,F,G,J-L) Germline clones of csn4^null (B,F,J) csn5^null (G,K) and csn5^p (C,L). csn5^null ovaries are essentially indistinguishable from csn4^null ovaries and are not shown. Staging of oocytes was according to position in ovariole.

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