Fig. 9. Gel filtration analysis of the COP9 signalosome and its subunits in the wild-type and mutant strains. Third instar larvae from wild-type and mutant strains were homogenized in gel-filtration buffer and total soluble protein was fractionated over a Superose 6 gel-filtration column (Pharmacia). Fractions (0.5 ml) were examined for the presence of CSN4, CSN5 or CSN7, as indicated, by immunoblot analysis with anti-CSN4, anti-CSN5 or anti-CSN7 affinity-purified antibodies. Positions of size markers are shown.

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