Fig. 1. Akap200 and Pka-RII localization in Drosophila wild-type ovaries. (A-C) Akap200 localization. (A) Akap200 protein was present on membranes of follicle cells and nurse cells at all stages of oogenesis. It was also a component of ring canals (arrows). (B) Akap200 expression diminished after stage ten in the follicle cells over the oocyte (arrow), but persisted in the nurse cell-associated follicle cells, nurse cells and ring canals. (C) Akap200 immunoreactivity (red) did not colocalize with Hu-li tai shao immunoreactivity (green). Akap200 was enriched on the outer rim of the ring canal (arrows). (D-F) Pka-RII localization. (D) Germarium and early-staged egg chambers. Like Akap200, Pka-RII protein localized to membranes and ring canals (arrows). (E) Later-staged egg chambers, including a portion of a stage nine egg chamber (arrowhead). Pka-RII was also enriched in a group of follicle cells at the anterior of egg chambers (bracket). (F) Pka-RII immunoreactivity (red) did not colocalize with Hu-li tai shao immunoreactivity (green), suggesting that it was enriched on the outer rim of ring canals.

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