Fig. 3. Expression of Brn3b (A,B) and SMA (C,D) in developing eyes. In situ hybridization using a ³⁵S-labeled riboprobe shows that Brn3b was specifically expressed in retinal ganglion cells in P1 wild type eyes (A). In P1 transgenic eyes, Brn3b transcripts were also detected in the region of the presumptive ciliary epithelium (B). Immunohistochemistry (C,D) shows that SMA was present in the wild-type developing iris (arrow in C) but its level was reduced in transgenic eyes (arrow in D). Morphologies of the inner (red arrowheads) and outer (blue arrowheads) layers of iris epithelia were altered in transgenic mice (D) when compared with the wild-type controls (C). Abbreviation: L, lens. Scale bars: 100 µm in A,B; 50 µm in C,D.

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