Fig. 2. LiCl treatment at gastrula mimics wnt8b overexpression. (A-D) Wild-type (control) embryos at 100% epiboly are stained with anterior neuroectoderm markers, opl, anf, six3 and fz8a. (E) Control embryo at 25 hpf. (F-I) One-cell embryos were injected with low dose of wnt8b mRNA (2 pg/embryo), further cultured to 100% epiboly and then their expression patterns of the anterior neuroectoderm markers analysed. (J) 25 hpf embryo injected with wnt8b. (K-N) Embryos were exposed to LiCl at the shield stage, allowed to grow until 100% epiboly and then their expression patterns of the anterior neuroectoderm markers were analysed. (O) 25 hpf embryo treated with LiCl at shield stage. Scale bars: in N, 250 µm for A-D,F-I,K-N; in O, 250 µm for E,J,O.

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