Fig. 4. Ectopic expression of fz8a reduces eye size, while Fz8a-CRDTM induces large eyes. Wild-type embryos (A,E) and embryos injected with fz8a (300 pg/embryo; B,F), fz8a-CRDTM (250 pg/embryo; C,G) and dNTCF3 (25 pg/embryo; D,H) were allowed to grow until the nine-somite stage. Changes of the eye size are shown in lateral view (upper panel) and anterior view (lower panel). Arrowhead indicates caudally reduced eye primordia in fz8a-injected embryos (F), and arrows denote the enlarged eye primordia in embryos injected with fz8a-CRDTM (G) or dNTCF3 (H). Scale bar: 250 µm. (I) Structure of cDNA constructs encoding dominant negative forms of mutant protein used for injection experiments. CRD, cysteine rich domain; TM, transmembrane domain; ß-BD, ß-catenin binding domain; NLS, nuclear localisation signal.

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