Fig. 7. Specificity of fz8a-MO and wnt8b-MO. The ability of fz8a-MO and wnt8b-MO, which can block dorsalisation induced by a co-injection of fz8a and wnt8b, were analysed at the bud stage embryos (A-D,G-J). Uninjected control embryos (A) and embryos dorsalised by a co-injection of fz8a (10 pg/embryo) and wnt8b (1 pg/embryo) mRNAs (B). Injection of fz8a-MO (14 ng/embryo) rescues the dorsalised phenotype induced by a co-injection of fz8a and wnt8b mRNAs (C). Injection of wnt8b-MO (13 ng/embryo) also rescues the dorsalised phenotype induced by a mixture of fz8a and wnt8b mRNAs (D). The expression of pax2 and no tail (ntl) in uninjected control embryos (G). The radialised pax2 expression and laterally expanded ntl expression caused by a co-injection of fz8a and wnt8b mRNAs (H) are rescued by fz8a-MO (I) and wnt8b-MO (J). The anterior view of the radialised expression of pax2 is shown in an inset (H). In control morpholino experiment, neither 4misfz8a-MO (E,F) nor 4miswnt8b-MO (K,L) could rescue dorsalised phenotype induced by co-injection of fz8a and wnt8b. Scale bar: in L, 250 µm for G-L.

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