Fig. 4. Comparison of LET-99 and PAR-3 staining in wild-type embryos. Confocal micrographs of wild-type embryos triple labeled with DAPI (A, E, I), anti-PAR-3 (B, F, J) and anti-LET-99 antibodies (C, G, K). Merged images (D, H, L) show PAR-3 in red, LET-99 in green and DNA in blue. (A-D) 1-cell embryo during pronuclear migration. (E-H) 1-cell metaphase embryo. (I-L) 2-cell embryo in which P1 is in late prophase. The focal plane was chosen to show the P1 cell most clearly. Scale bar: 10 µm.

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