Click on image to view larger version.

Fig. 6. Nuclear rotation fails in spherical par-3 one-cell embryos. DIC images of live wild-type (A-D) and par-3 (E-H) embryos during chitinase treatment to remove the eggshell. Arrowheads mark the position of the sperm pronucleus before nuclear migration. Asterisks indicate the centrosomes. In the par-3 embryo, the two centrosomes are aligned into the plane of the image, transverse to the axis defined by the position of the sperm, and thus only one centrosome is visible. Scale bar: 10 µm.