Fig. 4. Constitutive expression of MUK does not affect the radial glial fiber architecture. Adenovirus vectors were injected into the lateral ventricle of an E13 embryo and paraffin sections of the head region were prepared 3 days later, as in Fig. 3D-F. Sections were immunostained using anti-vimentin to visualize radial glial fibers (O’Rourke et al., 1992) (red). The expression of T7-MUK or T7-MUK/KR (green) and nuclear staining with DAPI (blue) are also shown. Cortical regions of the embryos infected with adenovirus vectors for T7-MUK (A) or T7-MUK/KR (B) or non-infected control embryo (C) are shown. Note that no significant changes in radial glial fiber architecture are induced by virus infection. The big yellow spots represent blood cells stained with the secondary antibodies. Arrows indicate radial glial fibers, and arrowheads indicate cells expressing T7-MUK or T7-MUK/KR. Asterisks indicate blood vessels with nonspecific staining of erythrocyte (yellow). Layer identifications are indicated at the bottom. Note the formation of the cortical plate in T7-MUK virus infected brain, as designated by a cluster of round nuclei. Scale bar: 100 µm.

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