Fig. 5. Comparison of endogenous and exogenous MUK-expressing cells. (A) A section of non-infected E16 embryo telencephalon was stained with anti-MUK antibody as in Fig. 1B, to represent endogenous MUK-expressing cells. Observation of the subventricular zone (SVZ) at higher magnification reveals numerous cellular processes (arrows) as well as cell bodies stained with anti-MUK antibody. (B) A section of a T7-MUK virus-injected embryo was prepared and stained as in Fig. 3D. Observation of the subventricular zone (SVZ) at higher magnification reveals numerous cellular processes (arrows), as well as cell bodies stained with anti-T7 antibody. (C) A section of non-infected E16 embryo telencephalon was stained with anti-T7 antibody to show the background staining level. (D) A section was prepared and stained as in A, except a secondary antibody conjugated with a fluorescent dye, Cy3 (red), was used to detect endogenous MUK expression. Nuclei were labeled with DAPI (blue). Immunofluorescent microscopy reveals the localization of the MUK protein at higher resolution, associated with a dot-like structure distributing close to or distant from nuclei (arrows). (E) A section of a non-infected E16 embryo was stained as in D, using anti-T7 antibody. Arrows indicate the dot-like distribution of the T7-MUK protein. (F) A section of non-infected E16 embryo was stained as in D, using anti-T7 antibody to show the background staining level. Scale bars: 20 µm.

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