Fig. 6. Migration arrested cells show features of immature neurons. (A) A section of non-infected E16 embryonic telencephalon was stained with anti-class III ß-tubulin antibody representing postmitotic immature and mature neurons (Menezes and Luskin, 1994). Note that most cells other than ventricular cells are stained. (B) A section of T7-MUK virus injected embryo was stained with anti-class III ß-tubulin antibody. The staining profile is indistinguishable from that of the non-infected embryo (A). (C) A close section was stained with anti-T7 antibody. As a large region of subventricular zone (SVZ)/intermediate zone (IZ) cells are expressing distinct levels of T7-MUK, these cells may also be expressing anti-class III ß-tubulin. (D) The expression of anti-class III ß-tubulin in T7-MUK-expressing cells is shown by double immunostaining. Dot-like signals for T7-MUK (red) are seen on cell processes or cell bodies, representing the presence of anti-class III ß-tubulin (green), as indicated by arrows. Nuclei were labeled with DAPI (blue). (E) A section of a T7-MUK virus injected embryo was double stained using anti-T7 antibody (red) and anti-MAP2 antibody (green) to show labeling of relatively mature neurons (Menezes and Luskin, 1994). Note the heavy staining of cells in the marginal zone (MZ), cortical plate (CP) and subplate (SP), and the relatively weak staining of cells in the subventricular zone (SVZ) and intermediate zone (IZ), including T7-MUK-expressing cells (arrows). (F) A section of T7-MUK/KR virus injected embryo was double stained as in E. The staining profile of MAP2 is essentially the same as in E, and in sections of non-infected embryo (data not shown). Arrows indicate T7-MUK/KR-expressing cells. Scale bars: 50 µm.

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