Fig. 1. Topographies of vertebrate and Drosophila Myb proteins. Schematic representations of the mouse and chicken c-Myb proteins and v-Myb protein from avian myeloblastosis virus (AMV; derived from the chicken gene) (Oh and Reddy, 1999). Abbreviations: R1, R2 and R3, three imperfect tandem repeats that comprise the DNA binding domain (region I); TA, transcriptional activator domain; LZ, leucine zipper; NR, negative regulatory domain. Also depicted is an additional region encoded by an alternatively spliced exon that contains the majority of conserved region II. The DNA-binding domain is also indicated. Regions of the c-Myb protein that are highly conserved between mouse and chicken are shown in the chicken protein in black. The four regions of conservation shared between vertebrate and Drosophila Myb proteins are indicated by Roman numerals (hatched in the DMyb proteins). The v-Myb protein has suffered both N- and C-terminal truncations, but the latter has been shown to be sufficient to activate transcriptional activation and transformation potentials of the Myb protein. Below are schematic representations of the two DMyb proteins produced by the fragments cloned into pUAST: DMyb, the full-length protein; and DMyb, the C-terminally truncated protein which is expected to be hyperactive by analogy to the v-Myb protein.

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