Fig. 1. Time-course of pEEBZ transgenic embryos stained for ß-galactosidase. (A) pEEBZ construct map. (B) Transgene expression starts before 8.5 dpc. At 9.5 dpc (C), epaxial somitic expression is evident in all somites and expression is also seen in the branchial arches (star) and in the head. At 10.5 dpc (D), dermomyotomal expression can be seen in the youngest somites. Transgene expression can be observed in the myotome in older somites where the dermomyotomal structure is breaking down. Additional ectopic expression can be seen in limbs (higher magnification inset). At 12.5 dpc (E), continued epaxial somitic expression and ectopic limb and head expression are seen.

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