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Fig. 7. Localisation of ß-galactosidase-positive cells within the myotomes of y200-Myf5-nlacZ and y200{Delta}E-Myf5-nlacZ 9.5 dpc embryos. (A-D) Transverse sections of y200-Myf5-nlacZ, (E-H) transverse sections of y200{Delta}E-Myf5-nlacZ. A and E are stained with Hoechst 33258 to show the anatomy. Desmin (red), used as a marker of myogenic cytodifferentiation, is detected throughout the myotome except in cells immediately adjacent to the DML (dorsomedial lip) (B,F). ß-galactosidase-positive cells (green) extend up to and within the epaxial DML in the y200-Myf5-nlacZ control sections (C) but are restricted to relatively ventral regions of the myotome in y200{Delta}E-Myf5-nlacZ (G). D and H show overlays (merged images) of B,C and F,G, respectively. White arrows mark the boundary of DML, red arrows mark dorsal desmin boundary, green arrows dorsal ß-galactosidase boundary.





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