Fig. 5. Leaf phenotypes resulting from dosage series. Aneuploidy experiments showing three sibling leaves with one copy of Rolled leaf1-O, but differing in dosage of the wild-type allele, rld1⁺: (left) Rld1-O/rld1⁺rld1⁺(hyperploid), (middle) Rld1-O/rld1⁺ (euploid), (right) Rld1-O/– (hypoploid). Rld1-O/rld1⁺rld1⁺ exhibits the least severe mutant phenotype. The abaxial ligule (black arrowhead) is present in only a narrow portion (as shown by size of black bar above ligule) of the blade width and the vascular disturbance is mild resulting in negligible ‘clearings’ (red arrowheads). Clearings result from a disturbed pattern of venation in the mutant (Fig. 3E). The width of the blade lamina is very similar to that observed in wild-type siblings for the same dosage series (data not shown). As rld1⁺ alleles change in dose from 2 to 0, all components of Rld1 phenes become more severe as can be seen for leaf-width and clearings.

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