Fig. 7. Leaf phenotypes of sectors from mosaic analysis. Transverse sections of Rolled leaf1 leaves containing non mutant white sectors. Plants are genotypically rld1⁺wlu4/ Rld1-OWlu4⁺ (Rolled leaf1 phenotype, green tissue). White tissue indicates removal of dominant mutant allele Rld1-O from particular tissue layers of sector. (A) Leaf with no white sectors as shown by inset cartoon. Macrohairs on the abaxial epidermis show the characteristic Rolled leaf1 phenotype. (B) White sector, indicated between arrowheads, marks the loss of Rld1-O. Close examination showed epidermal guard cells were still green, as shown by inset. This sector had the typical Rld1-O polarity as the presence of macrohairs on the abaxial epidermis in white tissue sector indicate. (C) Micrograph of abaxial epidermis with a sector running through it. To the left of the sector there are macrohairs (mh). No macrohairs are observed on this epidermis in the sector. (D) Transverse section of a sector where Rld1-O has been lost in all tissue layers except the abaxial epidermis (TL5) as shown by inset. Prickle hairs (ph) and bulliform cells (bc) are present on the abaxial epidermis. (E) Transverse section of a region of the sector seen in C. Prickle hairs and bulliform cells are present on the adaxial epidermis. (F) Adaxial epidermis of Rld1 sector seen in D, where Rld1-O was removed in all but the abaxial epidermis. Stomatal complexes (sc) contain guard cells that are white. (G) Abaxial surface of the region of sector in E marked by arrowheads. Rld1-O has been lost on this epidermis as observed in the stomatal complexes that are not fluorescing red. Inset cartoons show genotypes of each half of this sector. Tissue is phenotypically wild type. (H) Abaxial epidermis of same sector as F. Chloroplasts of guard cells (gc) are fluorescent red indicating presence of Rld1-O. The presence of macrohairs and prickle hairs on the abaxial surface indicate the sector is phenotypically Rolled leaf1.

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