Fig. 8. Migrating cardiac neural crest undergoes abnormal apoptosis in Fgf8^H/– mutants. Fluorescent immunohistochemistry was performed on transverse cryosections using a combination of anti-AP-2/FITC (green), TUNEL/Texas red (red) and either (A,B) anti-Tuj1/Cy5 (blue) which labels neuronal tubulin, or (C,D) antiPHH3/Cy5 (blue), which labels chromatin in proliferating cells. (A,B) Twenty-five somite stage matched (A) control and (B) mutant; plane and region of sections are illustrated by broken lines in Fig. 7B. White asterisks mark the third PAA in parallel. (C,D) Twenty-nine somite stage matched (C) control and (D) mutant; sections begin just posterior to the junction of the third aortic arch artery with the dorsal aorta (ao). From left to right, sections proceed from anterior to posterior through the post-otic region. Blue arrowheads indicate normal domains of apoptosis, including regions of developing ganglia and neural tube that label with anti-Tuj1. Yellow arrowheads indicate increased numbers of apoptotic cells in normal domains or abnormal domains of apoptosis in mutant neural crest mesenchyme. Yellow staining indicates double labeling of cardiac/pharyngeal neural crest cells with anti-AP-2 and TUNEL, and reveals abnormal apoptosis in lateral neural crest of the developing fourth arch. No reproducible differences in PHH3 staining were detected at this stage.

Return to article