Fig. 2. Expression of molecular markers of early gonadal development. (A-D) In situ hybridization of E12.5 XY gonads with Sf1 RNA probe. (A,C) Normal controls. (B) Fog2^–/–. (D) Gata4^ki/ki. Gonad (red arrow) and mesonephros (arrowhead) are indicated. (E-J) Analysis of SF1 (red) and WT1 (green) protein expression in E13.25 control XY gonads (E,H), control XX ovary (F,I) and XY Fog2^–/– gonad (G,J) using immunofluorescent histochemistry and confocal microscopy. Notice that the XY Fog2^–/– gonad lacks testis cords and resembles the control XX ovary at this stage of development.

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