Fig. 4. Blocking TAG-1 function in vitro alters the migratory behavior of LRN and ECN cells. Whole-mount in situ hybridization for TAG-1 on E12.5 explants after 2-4 days in culture. (A,C,E,G) Blocking the function of TAG-1 by cutting the GPI link with PI-PLC (A), blocking with Fab fragments of polyclonal antibodies against TAG-1 (C), or incubating with control non-depleted medium (E) or medium with soluble TAG-1-Fc (G). (B,D,F,H) Explants incubated in control medium (B), medium with control Fab fragments (D), in antibody-depleted medium (F) and in medium with control soluble protein MUC18-Fc (H). (I,J) Explants incubated in control medium for 2 days and then for 2 more days in medium with polyclonal antibody against TAG-1 (I) or with PI-PLC (J). The migration of TAG-1-positive cells occurred normally. Scale bars: 500 µm.
