Fig. 6. Chromosomal dynamics are disturbed during mitosis in mutant Dm myb abdominal histoblasts. Abdominal epidermal samples from females were doubly stained with DAPI to visualize nuclei (blue in merged panels) and with antibodies against the kinetochore associated protein, Bub1 (red in merged panels). (A-C) Mutations in Dm myb disturbed kinetochore arrangement during metaphase. (A) w/Df(1)sd72a control, 24 hours APF. (B) myb2, 42 hours APF. (C) The presence of more than 16 Bub1 signals in this myb2/Df(1)sd72a cell (42 hours APF) is indicative of polyploidy and demonstrates that such cells continue to progress into mitosis. Scale bar: 0.01 mm.
