Fig. 7. Aneuploidy, polyploidy and variable nuclear morphology in mutant Dm myb abdominal epidermal cells. (A,B) Abdominal epidermal samples from female pupae hybridized with an X-chromosome probe (red), and stained with DAPI (blue) and PH3 antibodies (green). (A) Two signals were seen in each of two separating nuclei in w/Df(1)sd72a controls during anaphase. (B) By contrast, a different number of signals (1, 4 and 6) were evident in each of three separating nuclei in a mutant myb2 cell. (C-K) Abdominal epidermal samples from females at 30 hours APF for controls and at 44-46 hours APF for Dm myb mutants were stained with DAPI for DNA quantitation (blue in D-K) and rhodamine-labeled phalloidin (red in D-K), and optically sectioned by confocal microscopy under identical conditions. For each of three experiments, nuclear fluorescent intensities were measured. The average value of the G1 control nuclei was determined and used as the base value of 1x. Values of other nuclei were adjusted accordingly to calculate relative fluorescent intensities. (C) Results of analyzing 121 control (w/Df(1)sd72a); 124 myb2, and 83 myb2/Df(1)sd72a nuclei are graphically represented. The range of values included in each category are indicated in parentheses as follows: 
0.5x ( 0.6); 1x (0.7-1.5x); 2x (1.6-2.5x) and 
3x ( 2.6). (D-K) Examples of control and mutant cells with relative DNA quantitation values as indicated. (D) Three control w/Df(1)sd72a G1 nuclei; (E) a control G2 nucleus is centered; (F) enlarged myb2 nucleus; (G) binucleate myb2 cell; (H) mis-shapen and enlarged myb2/Df(1)sd72a nucleus; (I) multilobed myb2/Df(1)sd72a nucleus; (J) a myb2/Df(1)sd72a nucleus with subdiploid DNA content (arrow); (K) a myb2/Df(1)sd72a cell containing a large nucleus and a micronucleus indicated by arrow. Scale bars: in A, 0.01 mm in A,B; in K, 0.01 mm in D-K.
