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Fig. 3. Differentiation of Ihh deficient embryoid bodies. (A) Day 10 Ihh–/– embryoid bodies fail to cavitate compared with Ihh+/– embryoid bodies. Ihh–/– embryoid bodies also appear to have increased levels of parietal endoderm (arrows). (B) Day 13 Ihh+/– embryoid bodies contain blood cells (stained with benzidine) and blood islands (arrows), whereas Ihh–/– consistently fail to cavitate or form blood island structures. Scale bars: 100 µm.





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