Fig. 3. Expression patterns of Notch signaling cascade components at E10. In situ RNA hybridization on cross-sections through the neural tube, dorsal to the left and ventral to the right, of E10 mutant (A,C,E,G) and control (B,D,F,H) embryos show an upregulation of the Notch ligands Dll1 (arrows, A) and Dll3 (arrows, C) in the medial aspect of the mutant cerebellar primordium. Mash1 (E,F) and Math1 (G,H) expression were also upregulated in the mutant cerebellar primordium compared to control littermates. (I,J) Whole-mount in situ RNA hybridization confirmed the ectopic expression of Math1 in the mutant compared with control embryos (arrow, I). Scale bars: in A, 100 µm for A-D; in E, 100 µm for E-H.
