Fig. 2. PAR localization. (A,B) PAR-2 in 4-cell (A) and 7-cell (B) embryos. (C,D) PAR-3 in early 4-cell (C) and 8-cell (D) embryos. (E) 28-cell embryo showing PAR-2 (green), PAR-3 (red) and DNA (blue); the ingressing E daughters are indicated with yellow asterisks. The embryo is oriented as in Fig. 4D. (F) Nomarski micrograph of 50-cell embryo indicating cell surfaces facing the blastocoel (arrowheads). (F') PAR-3 localization in embryo shown in F. PAR-3 is localized to surfaces facing the blastocoel (arrowheads) in addition to localization at the apical surface (arrow). (G) PAR-3 in an 8-cell ama-1(RNAi) embryo. (H) PAR-2 in an 8-cell par-3(it71) embryo; PAR-2 is localized to all cell surfaces (arrows). In A-E and G the germline precursor is indicated with a cyan asterisk.
