Fig. 1. brat expression pattern in brain and imaginal discs. Brains and imaginal discs were dissected from third instar larvae and hybridized in situ using a brat antisense RNA probe. (A) Brain with the ventral nerve chord to the left and the brain hemispheres to the right. (B) Antenna (left) and eye (right) disc. Black arrow, morphogenetic furrow; white arrows, neuronal preclusters. (C) Wing disc. (D) Leg disc. No signal was detected when the sense probe was used (not shown). Scale bars: 50 µm in A; 100 µm in B-D.
