Fig. 3. brat inhibits cell growth. (A) Part of a wing imaginal disc in which mitotic recombination was induced with a 2 hour heat shock at 72 hour AED to generate clones of cells that are brat¹¹/brat¹¹ (black areas) and GFP/GFP (bright green areas). Clones are surrounded by unrecombined brat¹¹/GFP cells (light green areas). Scale bar: 10 µm. (B,C) Flow cytometric analysis of dissociated wing imaginal discs containing control clones (B) or brat¹¹ clones (C). The x-axis shows forward light scatter, a relative measure of cell size, and the y-axis shows cell counts. Representative data from one experiment in which a 2 hour heat shock was applied at 72 hours AED is shown. (D) +/+ (control) or brat¹¹/brat¹¹ clone areas were compared with the corresponding GFP/GFP sister clones. A 45 minute heat shock was applied at 48 hours AED and clones were measured at 115 hours AED. Mean area ratio is plotted with standard error indicated by error bars. Data from two independent experiments was compiled. Genotypes are: hs-FLP¹²²; Ub-GFP FRT40A/brat¹¹ FRT40A (A, C and ‘brat¹¹’ in D) and hs-FLP¹²²; Ub-GFP FRT40A/FRT40A (B and ‘control’ in D).

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