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Fig. 7. brat overexpression inhibits cell division. Control, UAS-brat(1A) or UAS-brat(5) clones were induced at 72 hours AED and analyzed 43 hours later. The number of cells per clone was counted and plotted as a percentage of the total number of clones analyzed for each genotype. Doubling time (DT) is indicated next to each label. The statistical significances of the differences between experiments and control are: P=8x10–10 for UAS-brat(1A) and p=2x10–13 for UAS-brat(5). Number of clones analyzed was 76 for control, 104 for UAS-brat(1A) and 116 for UAS-brat(5). Genotypes are y w hs-FLP122; Sp/UAS-P35; Act>CD2>Gal4 UAS-GFP/+ (black bars), y w hs-FLP122; UAS-brat(1A)/UAS-P35; Act>CD2>Gal4 UAS-GFP/+ (white bars) and y w hs-FLP122; +/UAS-P35; Act>CD2>Gal4 UAS-GFP/UAS-brat(5) (striped bars). Similar results were obtained in two independent experiments.





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