Fig. 5. Differential expression of MB markers in the third instar brain. (A,D,G) Expression of dnc-lacZ (green/white). (B,E,H) GFP expression driven by 201Y (green/white). (C,F,I) GFP expression driven by c739 (green/white). (A-C) Dorsal views of the Kenyon cell clusters of wandering third-instar larvae labeled with an anti-DAC antibody (magenta/white). Single optical sections. Stars indicate the positions of the MB neuroblasts. (D-F) Lateral views showing the lobes and the distal part of the peduncles. Reconstruction of optical sections. (G-I) Single optical sections of the peduncles. The lobes and the peduncle are labeled with an anti-FAS II antibody (magenta/white). Note similar internal staining for the peduncles and the lobes. Expression of 201Y and c739 was monitored with UAS-GFP. FAS II expression is weak in the innermost axons labeled with c739 and absent in the core. (J) Lateral view of a third instar MB double labeled with anti-Synaptotagmin (SYT; magenta/white) and anti-ß-gal (green/white) antibodies. Reconstruction of optical sections. (K) High-magnification view of the dorsal lobe and heel showing a patchy distribution of the dnc-lacZ terminals in J. Single optical section. (L) A high power view of the dendritic arborization (J) of the dnc-lacZ neurons in the calyx. Note the extensive arborization of the dnc-lacZ neurons around the globular synaptic terminals (arrowheads). Single optical sections. Scale bars: in A, 25 µm for A-C; in D, 50 µm for D-F; 50 µm in J; 20 µm in K,L. ACT, antennocerebral tract; cx, calyx; DL, dorsal lobe; h, heel; ML, medial lobe; Ped, peduncle.
