Fig. 5. Sox9-AS prevents Slug expression in a dose-dependent manner. Embryos were injected in one blastomere at the two-cell stage with different concentration of Sox9-AS and analyzed for Slug expression by whole-mount in situ hybridization at stage 17. (A-C) Representative embryos illustrating unperturbed Slug expression (A) and partial (B) or complete (C) loss of Slug on the injected side. Dorsal view, anterior is at the top. RNA encoding the lineage tracer ß-galactosidase was co-injected with Sox9-AS to identify the injected side (red staining); the left side in all panels. (D) Quantification of Slug in situ hybridization results. (E) Early onset of Slug expression (arrow) at the late gastrula stage (stage 12) is blocked in embryos injected with 10 ng of Sox9-AS. Dorsal view, anterior is at the top, injected side (red staining) is to the left.
