Fig. 5. Defects in pop-1(Sys) and pop-1(RNAi) male gonads. All animals carry qIs56[lag-2::GFP], which expresses brightly in the LC and weakly in DTCs and other somatic gonadal cells. LC, linker cell; SPm, somatic primordium of male; DTC, distal tip cell. Extents of SPm and germline are indicated by brackets. (A,B) Wild-type L2 male. Single LC leads migration of the single gonadal arm. The SPm resides directly behind the LC and follows its migration. Two DTCs are present, but only one is seen in this focal plane (arrowhead). (C,D) pop-1(q645) male in L2 lethargus or early L3. Two LCs are present. The SPm appears normal: the somatic gonadal cells cluster to the anterior and follow the migrating LC. Only one DTC is present (arrowhead). (E,F) pop-1(RNAi) L2 male. Gonad polarity and organization are normal: the SPm is established at the anterior directly behind the LC. However, no DTCs are present and the LC has not migrated. The lack of LC migration is accompanied by a widening of the developing gonad.
