Fig. 5. Expression of Pax6 and Numblike (Nbl) in wild-type and Pax6^Sey/Sey cortex. In all sections dorsal is upwards and in colour images nuclei are counterstained with DAPI (blue). (A-F) Coronal sections through (A-C) E10.5 wild-type and (D-F) E10.5 Pax6^Sey/Sey brains. (G-L) Parasagittal sections through (G-I) E15.5 wild-type and (J-L) E15.5 Pax6^Sey/Sey brains. (A,D,G,J) Low-magnification images of (A,G) wild-type and (D,J) Pax6^Sey/Sey brain sections stained with DAPI. Boxes show areas in the cortex in which images to right were taken. (B,E,H,K) Nuclear Pax6 expression (green) is detected in wild-type ventricular zone (B,H) but not in the mutant (E,K). (C,F,I,L) Weak cytoplasmic Nbl expression (green) is detected in a few cells in E10.5 wild-type preplate (PP) (C). The Nbl expression domain has expanded in the wild-type cortex by E15.5 (I): expression is observed in both the wild-type intermediate zone (IZ) and cortical plate (CP), although expression levels appear to be much stronger in the IZ compared with the CP. (F,L) In the mutant, more Nbl-positive cells are detected in the preplate at E10.5 (F) compared with wild type (C). Nbl is highly expressed by cells in the Pax6^Sey/Sey IZ and CP at E15.5 (L). The Nbl expression appears higher in the mutant CP compared with wild-type levels. c, cerebral vesicle; d, diencephalon; SVZ, subventricular zone, VZ, ventricular zone. Scale bars: 100 µm in A,D; 10 µm in B,C,E,F,H,I,K,L; 200 µm in G,J.

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