Fig. 7. Histone H3 lysine 9 methylation in hermaphrodites. N2 (Bristol) (A,C) and transgenic strain PD7271 (B) hermaphrodite ovaries were fixed in methanol/acetone and stained with anti-H3 dimethyl-K9 antibody (green) and counter-stained with DAPI (red). The progression of the nuclei through meiotic prophase I is indicated by labels and arrows in A. Arrowheads in B indicate the major staining foci seen in early pachytene nuclei in all strains; the arrows show additional nuclear foci unique to the PD7271 strain, which correspond to the transgene arrays. (C) An enlargement of the pachytene-to-diplotene transition region from A. Scale bars: 30 µm in A; 5 µm in B,C.
