Fig. 9. In situ analysis of X-linked oocyte gene expression. (A-C) Antisense probes were generated for several X-linked genes that exhibited an oocyte-enriched profile by microarray analysis (Reinke et al., 2000). The probes were then hybridized to wild-type ovaries to determine where the corresponding mRNAs begin to accumulate. In each panel, the mitotic (distal) region is towards the left and maturing oocytes in the most proximal region of each ovary are towards the right. The arrowheads indicate the boundaries of the pachytene region of each ovary. DAPI and lacZ are shown separately in A; these signals have been merged in B,C. (D,E) Distribution of transcription-competent RNA POL II. A methanol/acetone fixed hermaphrodite gonad was stained with DAPI (D) and also with monoclonal antibody H14 (E).
